Original Research Article | OPEN ACCESS
Preliminary Growth Inhibitory Studies of the Methanol Extract and Fractions of Leaves of Artocarpus altilis.

Emmanuel O Ikpefan

Department of Pharmacognosy and Traditional Medicine, Faculty of Pharmacy, Delta State University Abraka.;

For correspondence:-     Email: ikpefanemmanuel@delsu.edu.ng   Tel:+2348062366928

Published: 29 December 2018

Citation: Ikpefan EO. Preliminary Growth Inhibitory Studies of the Methanol Extract and Fractions of Leaves of Artocarpus altilis.. J Sci Pract Pharm 2018; 5(2):275-280 doi:

© 2018 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: Artocarpus altilis is one of the plants used by herbalists in parts of Abraka, Delta State for the management of various forms of cancer. This work is aimed at evaluating this claim using bench-top assay involving anti-proliferation of seed radicles of Sorghum bicolor.

Methods: The powdered leaf sample was extracted with 90% methanol by cold maceration and the extract was evaluated for the presence of phytochemicals. The extract was subjected to vacuum liquid chromatographic fractionation and the fractions were all subjected to biological screening using guinea corn radicle inhibitory assay at concentrations between 1-30 (crude extract) and 1-10 mg/mL (fractions)  respectively, over a period of 96 h.

Results: The extract showed the presence of alkaloids, saponins, tannins, cardiac glycosides, flavonoids and anthraquinones. The controls had an average length of 32.45±5.70 mm, whereas the seeds treated with 20 mg/mL and 30 mg/mL of the crude extract had an average length of 2.03±0.87 mm and 0.40±0.28 mm respectively, indicating 93.70 and 98.77% reduction in length respectively. The bulked vlc fraction A4 was observed to exhibit a higher inhibitory effect over A3 and A10 bulked fractions as it gave an average of 0.65±0.57 at 10 mg/mL after 96 h indicating 99% reduction in length compared to 46.45 ± 5.32 mm of the control.

Conclusion: This study involving the use of seed radicle assay to evaluate the inhibitory potential of this plant has to an extent validated the traditional use of this plant in the management of various forms of cancer. However, the need to isolate the active ingredients responsible for the observed activity and their corresponding biological activities on human cancer cell lines will form the basis of the next phase of this work

 

  

 

 

Keywords: Sorghum bicolor, phytochemicals, seed radicle, vacuum liquid chromatography


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