Loretta O Iniaghe1
Agatha I Onyemaonyeoru2
1Department of Pharmacology & Toxicology, University of Benin, Nigeria;
2Department of Science Laboratory Technology, University of Benin, Nigeria.
For correspondence:- Loretta Iniaghe
Published: 31 December 2015
Iniaghe LO, Onyemaonyeoru AI.
Evaluation of the analgesic property of the ethanolic extract of Garcinia kola Heckel (Guttiferae) seeds in mice. J Sci Pract Pharm 2015; 2(1):46-50
© 2015 The author(s).
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: Garcinia kolaHeckel (Guttiferae) is used in Ethnomedicine for the management of pain, respiratory infections, inflammation and food poisoning. This study was undertaken to evaluate the analgesic property of the ethanolic extract of Garcinia kola seeds in mice.
Methods: The oral lethal dose (LD50) of ethanolic extract of Garcinia kola seeds was estimated and the extract was screened for analgesic activity orally using the hot plate and acetic acid-induced mouse writhing tests in mice at graded doses of 100, 200 and 400 mg/kg and compared with standard analgesic agents- Acetylsalicylic acid and Morphine.
Results: The LD50 of Garcinia kola was estimated to be more than 5000 mg/kg. In the acetic acid-induced test, a significant (p<0.05) reduction in the number of writhes compared to control animals was observed at all doses of the extract (control, 45.73 ± 3.6;100mg/kg 24.98 ± 2.48; 200mg/kg 20.08 ± 5.59; 400mg/kg 16.39 ± 4.25 and Acetylsalicylic acid 6.97 ±2.87). Similarly, a significant increase (p<0.05) in the latency time spent on the hot plate at different intervals post treatment ranging from 59.62 to 81.67%, 108.37 to 156.26 and 48 to 78.04% respectively for 100, 200 and 400mg/kg dose levels of Garcinia kola was recorded in animals used for this study. Latency periods for control groups were not significantly increased (5.83 to 25.77%) while Morphine treated animals gave an increase in latency time of 122.76 to 158.29%.
Conclusion: Garcinia kola seeds possess analgesic property which may account for their use in Ethnomedicine.
Garcinia kola, hot plate, acetic acid, mouse writhing, pain