Josephine O Ofeimun
Buniyamin A Ayinde
Department of Pharmacognosy, Faculty of Pharmacy, University of Benin, University of Benin, PMB 1154, Benin City, 300001, Nigeria;
For correspondence:- Josephine Ofeimun
Published: 31 December 2017
Ofeimun JO, Ayinde BA.
Preliminary investigation of the aphrodisiac potential of the methanol extract and fractions of Rhaphiostylis beninensis Planch ex Benth (Icacinaceae) root on male rats. J Sci Pract Pharm 2017; 4(1):182-188
© 2017 The author(s).
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: The study investigated the claimed use of the root of Rhaphiostylis beninensis (Rb) as an aphrodisiac.
Methods: The methanol extract of the root of Rb as well as the chloroform and aqueous fractions were administered to male rats (100, 200, 400 mg/kg), which were mated with female animals that had been brought into oestrus by the sequential administration of 100 µg/animal of ethinyl oestradiol orally 48 h before and progesterone (1.0 mg/animal) subcutaneously, 6 h prior to mating. Tween 80 (5 %) and 50 mg/kg sildenafil served as the negative control and positive control respectively. Parameters determined were; mounting latency (ML), intromission latency (IL), ejaculation latency (EL), mounting frequency (MF), intromission frequency (IF), ejaculation frequency (EF), anogenital grooming (AG), genital sniffing (GS).
Results: The methanol extract and aqueous fraction were observed to show a dose dependent increase in MF, IF, EF, AG and GS, while a dose dependent decrease in ML, IL and EL was observed. The chloroform fraction showed the same pattern of dose dependent activity for all the parameters evaluated except for the IL, where the effect produced by 200 mg/kg was higher than that produced by 400 mg/kg dose.
Conclusion: This study provides evidence for theuse of the root of Rb in ethno-medicine as an aphrodisiac
Male sexual dysfunction, impotence, ethno-medicine, chloroform and aqueous fractions