Original Research Article | OPEN ACCESS

Phytochemical screening, antiproliferative and apoptotic activities of the root bark extract and fractions of Cola rostrata (Sterculiaceae) K. Schum. in MCF-7 cell line

Emmanuel E Odion1 , Nanja E Lutz2, Abiodun Falodun1, Sunday A Adelusi1,

1Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Benin, Benin City, Edo State, Nigeria; 2University of Rostock Medical Faculty, Department of Cell Biology, Schillingallee 69, 18057 Rostock, Germany.

For correspondence:-  Emmanuel Odion   Email:  emmanuel.odion@uniben.edu   Tel:  +2349076629635

Published: 11 August 2020

Citation: Odion EE, Lutz NE, Falodun A, Adelusi SA, Phytochemical screening, antiproliferative and apoptotic activities of the root bark extract and fractions of Cola rostrata (Sterculiaceae) K. Schum. in MCF-7 cell line. J Sci Pract Pharm 2019; 6(1):337-343 doi: https://doi.org/10.47227/jsppharm/v6i1.9

© 2019 The author(s).
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..


Background: Some Cola species are used in folklore medicine for the management of cancer related illnesses; thus, the objective of this work was to determine the phytochemical constituents, anti-proliferative and apoptotic activities of crude extract and fractions from the root bark of Cola rostrata on MCF-7 cells line.

Methods: The root bark of Cola rostrate was collected, identified, cleaned, air dried, pulverized, extracted with methanol and fractionated into petroleum ether, chloroform, ethylacetate and butanol and these fractions were concentrated in vacuo. The MCF-7 cells lines were treated with 10 µg/ml of the crude extract and fractions of Cola rostrata.

Results: The cells cycle progression showed that the crude extract had an apoptotic activity with 15.96 % of cells undergoing apoptosis as compared to 6.26 % of cells treated with DMSO, showing 9.70 % increase in population of apoptotic cells (P < 0.001). Also chloroform fraction treated MCF-7 cells gave 30.30 % proliferation which showed a significant decrease when it was compared with DMSO treated cells of 39.34 %, indicating 9.04 % reduction in proliferative cells (P < 0.01).

Conclusion: These results provide evidence that Cola rostrata crude extract induces antiproliferative action, while the chloroform fraction induces apoptotic activity in MCF-7 cell line and could be used in the management of MCF-7 cell line ailments.



Keywords: Cola rostrata, MCF-7, Flow cytometer, Apoptosis, Antiproliferation

Journal Metrics
Impact Factors
» Thompson Reuters (ISI): 0.000 (2020)
» H-5 index: XX (2020)
» SCImago Journal Rank (SJR): 0.000

» Submission to first review: 07-14 days
» Submission to acceptance: 20-30 days
» Acceptance to publication: 20-30 days

Article Tools

Share this article with


2019; 6: 
2018; 5: 
1, 2.
2017; 4: 
2016; 3: 
2015; 2: 
2014; 1: